Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures
نویسندگان
چکیده
منابع مشابه
Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures
Nanomanipulation under scanning electron microscopy (SEM) enables direct interactions of a tool with a sample. We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus. In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescenc...
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These days, cellular and molecular biology are closely integrated due to the development of novel correlative biomolecular microscopy platforms, even though this avant-garde vision of combining different imaging tools has been around since the late 1960s. The current boom and successful application of correlative imaging methods to dissect the cell’s internal structure is progressing faster tha...
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Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving ...
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ژورنال
عنوان ژورنال: Light: Science & Applications
سال: 2014
ISSN: 2047-7538
DOI: 10.1038/lsa.2014.105